关键词:
卵形疟； 输入性病例； 镜检； 快速诊断检测； 巢式PCR
Abstract: Objective Objective To diagnose the first imported case of Plasmodium ovale infection by laboratory detection. Meth Meth? ?
ods ods The epidemiological data and blood samples of the case were collected，and the samples were detected by the microscopic
examination，rapid diagnostic test（RDT）and nested PCR. Results Results The patient was a construction worker backing from Con?
go，Africa. He experienced the symptoms of irregular fever and weakness one month after returning in Lingyang Town，Junxian
County. The results of RDT only suggested no?Plasmodium falciparum infection. Under the microscope，it was seen that the in?
fected RBC were obviously disfigured and in irregular shape，the ring forms were thick and big，and also thick granulas in big
trophozoite stage and schizont stage were found. The results of PCR showed that the size of amplified product was about 800 bp，
which was conformed to that of P. ovale. Conclusion Conclusion Though microscopic examination is the golden standard for malaria diag?
nosis，as P. ovale is difficult to be identified under microscope，the microscopic method combined with PCR test can be used for
definite diagnosis.
Key words:
Plasmodium ovale；Imported case；Microscopy；Rapid diagnostic test；Nested PCR